Fig. 1.

Inhibiting Cdk5 increases Src activity. a Human lens epithelial cells were incubated with or without roscovitine. Cells were lysed and immunoblotted with antibodies against Src(pY419), Src, Cdk5, and GAPDH. b Cells were transfected with dominant negative Cdk5(D144 N), Cdk5, or a GFP control plasmid, and immunoblotted as in a. c Cells were transfected with siRNA oligonucleotides to suppress endogenous Cdk5 (siCdk5), or with non-targeting oligonucleotides (siControl). Cell lysates were immunoblotted as in a. d Cells were transfected with siRNA-resistant Cdk5 rescue plasmid (rCdk5), a small hairpin plasmid to suppress endogenous Cdk5 (shCdk5), or shCdk5 plus rescue plasmid Cdk5, or were mock-transfected (Mock). Cell lysates were immunoblotted as in a