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. Author manuscript; available in PMC: 2012 Sep 16.
Published in final edited form as: Vaccine. 2011 Jul 27;29(40):7080–7089. doi: 10.1016/j.vaccine.2011.07.008

Figure 2. An Ad5 encoding firefly luciferase efficiently penetrates the sublingual mucosa following SL instillation, while a HSV-1 amplicon vector encoding a matched insert fails to do so.

Figure 2

An Ad5 vector encoding firefly luciferase (1 × 108 VP), or a HSV-1 amplicon vector (5 × 105 VP) encoding the same insert was administered via the IM or SL route to female BALB/c mice; control animals were inoculated with PBS. 24 hours after administration animals were anesthetized with Avertin (240 mg/kg), and then injected I.P. with D-Luciferin (250 mg/kg), prior to imaging 10 minutes later using a Xenogen IVIS system to quantitate luciferase expression. Data were then analyzed using Living Image Software and normalized based on background luminescence. Results represent mean values from 4 animals/group; bars denote the standard error of the mean. Data are representative of 3 experiments for each vector/condition. *: Denotes a statistically significant difference in luciferase expression levels for sublingually delivered Ad5:Luc versus HSV-1:Luc (p=0.0286, Mann Whitney test).