Fig. 2. Characterization of human b12 iHVs.
a. Schematic drawing of human intermediate b12 scFv library. b. AA sequence alignment of the isolated human intermediate b12 HVs (iHVs) with human germline (Hub12-gHV) and mature (Hub12-mHV) HVs. c–d. Binding (c) and neutralization activities (c & d) of human b12 iHVs when recombined with human mature D(J)-segments linked to mature VL (-m) or with human germline D(J)-segments linked to human germline VL (-g). Human mature and germline b12 scFvs were included for comparison.