Figure 4.

cdc6-3 mutant cells accumulate more than 2C DNA. ρ° strains of W303-1A (thin lines and shaded area) and cdc6-3 (thick lines and unshaded area) were grown asynchronously in YPD at 25°C (YPD/Asy/25°C), blocked in G₁ with α factor (α factor/25°C), and then released into fresh YPD at 37°C (A), or at 25°C and then nocodazole (Noc) was added 60 min postrelease (B). Aliquots of cells were taken at various times after the release and analyzed by FACS. DNA contents of 1C and 2C, representing G₁ and G₂ cells, respectively, are marked. The peaks very close to the vertical axis (no DNA) are attributable to broken (from sonication) and dead cells, mainly of the mutant.