Figure 8.

 The CDK activities in S and G₂ in cdc6-3 are as high as in the wild-type cells. W303-1A and cdc6-3 cells were grown asynchronously in YPD at 25°C (Asy), synchronized in G₁ with α factor (0 min) and then released into fresh YPD at 25°C. Samples were taken at various times (20–120 min) after the release. The last lanes (Noc) were from cells incubated in YPD containing nocodazole at 25°C for 4 hr. Native proteins were extracted and used for p13 bead precipitation. The precipitates were assayed for histone H1 kinase activities.