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ASE is essential and sufficient for L-R asymmetric expression of nodal. (A) Structures and activities of the lacZ constructs 3′-1, 3′-1ΔASE, 5′-1ASE, and 501/301hsp. (B) X-gal staining patterns of representative transgenic embryos. Constructs 3′-1, 5′-1ASE, and 501/301hsp yielded left-sided staining in the LPM, whereas 3′-1ΔASE did not. Construct 501/301hsp also gave rise to left-sided staining in PFP.
