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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: Cell Immunol. 2011 Jun 23;271(1):124–133. doi: 10.1016/j.cellimm.2011.06.012

Fig. 1. Gating strategies for cell sorting of CFDA-SE positive macrophage cells and effects of in vitro culture and collagenase treatment on C2D macrophage phenotype.

Fig. 1

A) C2D macrophage cells were sorted based on negative expression of CFDA-SE, B) C2D CFDA-SE macrophage cells were sorted based on positive CFDA-SE expression; C) Example of C2D CFDA-SE+ cells that were sorted from a mixed cell sample such as C2D CFDA-SE+ macrophages (region 2) co-cultured with 3T3L1 adipocytes (region 1); D) PEC-C2D macrophages were treated with isotype control antibody (top) or anti Mac-2 antibody (middle and bottom) then assessed by flow cytometry. Cells were treated with PBS (middle) or collagenase (bottom) for 40 minutes before antibody probing.