Figure 2.

Identification of C/EBPα and C/EBPβ translation initiation sites. Schematic representations of the C/EBP mRNAs with the initiation sites indicated in relation to the protein bands are shown at left. Wild-type cDNA (wt), constructs that lack distinct translation initiation codons (ΔA, ΔB1, ΔB2, ΔC, and ΔD) or an amino-terminal deletion construct (C) were transiently transfected in COS-1 cells. C/EBP protein expression was analyzed by immunoblotting of total cell extracts: (a) rC/EBPα; (b) cC/EBPα; (c) rC/EBPβ; (d) cC/EBPβ.