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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: Cell Immunol. 2011 Jul 18;271(1):110–117. doi: 10.1016/j.cellimm.2011.06.010

Fig. 5. Treg cell sorting.

Fig. 5

CD4+ T cells were isolated from PBMC by magnetic cell separation, and then labeled with CD25-PE mAb. CD25+ and CD25− lymphocyte populations were sorted by FACS. (A) Pre-sort gates for CD25+ (red, Treg) and CD25− (blue, Tresp) populations are shown. (B) Post-sort purity analysis of CD25− (blue) and CD25+ (red) sorted populations. The post-sort CD25+hi population had 99.1% purity. Representative results from 1 of 6 subjects.