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. 2011 Jul 1;34(3):396–405. doi: 10.1590/S1415-47572011005000018

Figure 3.

Figure 3

The effects of 2F2R deletions on GFP gene expression. (A) Positions and sequences of the deletion mutations in 2F2R. (B) Single fragments or double tandem fragments of 2F2R and deletions (45R, 30R, 22R, 19R and 16R) were inserted downstream of the GFP gene in pAlu14. HeLa cells were transfected with the expression vectors and GFP RNA was detected by northern blotting. (C) Nucleotide sequences of Poly4 and Secloop and their double tandems. The nucleotides linking two fragments are underlined. (D) Poly4 and Secloop and their double tandems were inserted downstream of the GFP gene in pAlu14. HeLa cells were transfected with the expression vectors and the GFP RNA was detected by northern blotting.