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. 2011 Jul 1;34(3):396–405. doi: 10.1590/S1415-47572011005000018

Figure 8.

Figure 8

The effects of 22R mutants on GFP gene activation. (A) The 22R DNA sequence is predicted to form an imperfect stem-loop structure that includes a loop (3 nt), an initial stem (3 bp), a bulge (2 nt) and a second stem (3 bp). (B) Nucleotide sequences of 22R and its mutants. Predicted loop bases are underlined. (C) The double tandems of 22R and mutants of this fragment were inserted downstream of the GFP gene in pAlu14. HeLa cells were transfected with the expression vectors and GFP RNA was detected by northern blotting.