Table 1.
Primers used to construct the expression vectors.
| Primer identification | Sequence | Annotation |
|---|---|---|
| 1F (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAATGCTTACAATTTACGCGTTA-3′ |
Amplifying 1F1R, PolyAas |
| 1R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCTGCATTCTAGTTGTGGTT T -3′ |
Amplifying 1F1R |
| Poly60-2F (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAAGTGAAAAAAATGCTTTATT -3′ |
Amplifying 2F2R,45R,30R |
| Poly60-2R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCATAATGGTTACAAATAAAG -3′ |
Amplifying 2F2R, Poly4 |
| 3F (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAAAAGC TGCAATAAACAAGTT-3′ |
Amplifying 3F3R |
| 3R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCCCCTGA ACCTGAAACATAA-3′ |
Amplifying 3F3R, 3R49, 3F235 |
| 4F (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAAGGAGGTGTGGGAGGTTTTT-3′ |
Amplifying 4F4R |
| 4R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCTAATCAGCCATACCACATT-3′ |
Amplifying 4F4R, PolyAas |
| PolyAasF (forward primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCTGCTAATGCTTACAATTTACGCGTTA-3′ |
Amplifying PolyA |
| PolyAasR (reverse primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAATCAGCCATACCACATT-3′ |
Amplifying PolyA |
| FirLoopF (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAATGTGAAAAAA |
Amplifying 22R, 19R, 16R, 0 nt, 1 nt, 2 nt, 4 nt, 5 nt, 6 nt, TCC, GTC, GCA, CTC, GGC |
| FirLoopR (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCACAAATAA |
Amplifying 0 nt, 1nt, 2 nt, 4 nt, 5 nt, 6 nt, TCC, GTC, GCA, CTC, GGC |
| 1619MR (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAC-3′ |
Amplifying 19R, 16R |
| Poly45R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAAAGCAATAGCATCA-3′ |
Amplifying 45R |
| Poly30R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCCAAATTTCACAAATA-3′ |
Amplifying 30R |
| SecloopF (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAATGCTTTATTTGT-3′ |
Amplifying Secloop |
| SecloopR (reverse primer) |
Kpn I Nhe I 5′ATCGGGTACCATGCTAGCCACAAATTTCAC-3′ |
Amplifying Secloop |
| Poly4F (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTTAATCTAGATAATGATGCTATTG-3′ |
Amplifying Poly4 |
| EcoXba (forward primer) |
EcoR I Xba I 5′-ATCGGAATTCTT AATCTAGA-3′ |
Amplifying 17ntAT |
| KpnNhe (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGC-3′ |
Amplifying 17ntAT |
| 3F46F(forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAAAGCTGCAATAAACAAG |
Amplifying 3F46 fragment with reverse primer 3F135R |
| 3R49F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAAACAAGTTAACAACAA |
Amplifying 3R49 fragment with reverse primer 3R; Amplifying 3F135 fragment with 3F135R |
| 3F135R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCCATAAAATGAATG -3′ |
Amplifying 3F135, 3F46 |
| 3F235F(forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAAACAATTGCATTC-3′ |
Amplifying 3F235 fragment with reverse primer 3R |
| 3F46d2F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAGCTGCAATAAACAAG |
Amplifying 3F46d2 fragment with reverse primer 3F135R |
| 3F46d3F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGACTGCAATAAACAAGT-3′ |
Amplifying 3F46d3 fragment with reverse primer 3F135R |
| 3F46d4F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGATGCAATAAACAAGTT-3′ |
Amplifying 3F46d4 fragment with reverse primer 3F135R |
| 3F46d5F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAGCAATA AACAAGTTA-3′ |
Amplifying 3F46d5 fragment with reverse primer 3F135R |
| 3F46d6F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGACAATAAACAAGTTA-3′ |
Amplifying 3F46d6 fragment with reverse primer 3F135R |
| 3F46d7F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAAATAAACAAGTTA-3′ |
Amplifying 3F46d7 fragment with reverse primer 3F135R |
| 3F46d8F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAATAAACAAGTTA AC-3′ |
Amplifying 3F46d8 fragment with reverse primer 3F135R; Amplifying 3F38d1, 3F38d2, 3F38d3, 3F38d4, 3F38d5, 3F38d6, 3F38d8, 3F38d9, 3F38d10, 3F38d11, 3F38d12, 3F38 d13 with corresponding reverse primers |
| 3F46d9F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGATAAACAAGTTAACA-3′ |
Amplifying 3F46d9 fragment with reverse primer 3F135R |
| 3F46d10F (forward primer) |
HindIII Xba I 5′-ATCGAAGCTTAATCTAGAAAACAAGTTA-3′ |
Amplifying 3F46d10 fragment with reverse primer 3F135R |
| 3F38d1R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCATAAAATGAATGCA-3′ |
Amplifying 3F38d1 fragment with forward primer 3F46d8F |
| 3F38d2R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCTAAAATGAATGCAA-3′ |
Amplifying 3F38d2 fragment with forward primer 3F46d8F |
| 3F38d3R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAAAATGAATGCAAT-3′ |
Amplifying 3F38d3 fragment with forward primer 3F46d8F |
| 3F38d4R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAAATGA TGCAATT-3′ |
Amplifying 3F38d4 fragment with forward primer 3F46d8F |
| 3F38d5R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAATGAATGCAATTG-3′ |
Amplifying 3F38d5 fragment with forward primer 3F46d8F |
| 3F38d6R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCATGAATGCAATTG-3′ |
Amplifying 3F38d6 fragment with forward primer 3F46d8F |
| 3F38d8R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCGAATGCAATTG-3′ |
Amplifying 3F38d8 fragment with forward primer 3F46d8F |
| 3F38d9R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCAATGCAATTG-3′ |
Amplifying 3F38d9 segment with forward primer 3F46d8F |
| 3F38d10R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCATGCAATTG-3′ |
Amplifying 3F38d10 fragment with forward primer 3F46d8F |
| 3F38d11R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCTGCAATTG-3′ |
Amplifying 3F38d11 fragment with forward primer 3F46d8F |
| 3F38d12R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCGCAATTGTTGTTGTTAACTT-3′ |
Amplifying 3F38d12 segment with forward primer 3F46d8F |
| 3F38d13R (reverse primer) |
Kpn I Nhe I 5′-ATCGGGTACCATGCTAGCCAATTGTTGTTGTTAACTT-3′ |
Amplifying 3F38d13 fragment with forward primer 3F46d8F |
Underlined sequences indicate restriction enzyme cleavage sites.