Figure 6.

FIN219 mRNA expression and its auxin regulation. (A) FIN219 expression is reduced in light-grown fin219 mutants. Equal amounts of total RNA from 7-day-old dark- and light-grown wild-type (wt) and fin219 mutant (mut) seedlings were loaded in each lane. (B) Time course of expression of FIN219 mRNA induction by auxin. Seven-day-old seedlings grown in light were transferred to a liquid medium in the presence of 10 μm NAA. (C) Similar auxin induction test as in B with 2 or 4 hr after addition of auxin. The effect of 70 μg/ml cycloheximide and NAA alone, or together, was tested.