Skip to main content
. 2000 Aug 1;14(15):1958–1970.

Figure 7.

Figure 7

FIN219 is a cytoplasmic protein. (A) Localization of FIN219 in onion cells in a transient assay. Constructs encoding either GUS–FIN219 (a,b), GUS–NIa (c,d), or GUS–COP1 (e,f) were introduced into onion cells by particle bombardment. The onion cells were incubated in complete darkness (a,c,e) or under continuous white light (b,d,f) after bombardment for 2 days, stained for GUS activity, and then mounted whole in the presence of DAPI. The bottom each panel shows DAPI staining of the same cells to visualize the position of the nucleus (arrow). Scale bars, 100 μm in a–f. (B) Localization of FIN219 in stable transgenic plants. GUS–FIN219 (a,b) and GUS–NIa (c,d) staining patterns in root tips are shown. (a,c) Dark-grown seedlings; (b,d) light-grown seedlings. The bottom of each panel shows DAPI staining of the same field to visualize the position of the nucleus (arrow). Scale bars, 100 μm in a–d.