Table 1. Complete list of primers used in this study.
Capital letters indicate a locked nucleic acid (LNA). Ambiguous bases: K = G or T, M = A or C, R = A or G.
| Primer | Sequence (5′–3′) |
| Cloning experiment | |
| Forward (4F)* | ttg-gag-agt-ttg-atc-mtg-gct-c |
| Reverse (pH/1542r)† | aag-gag-gtg-atc-cag-ccg-ca |
| Universal PCR | |
| Forward (4F) | ttg-gag-agt-ttg-atc-mtg-gct-c |
| Reverse (pD)† | gta-tta-ccg-cgg-ctg-ctg |
| Universal cycle sequencing reactions | |
| Forward (Bact-8S-20)‡ | aga-gtt-tga-tcm-tgg-ctc-ag |
| Reverse (pD) | gta-tta-ccg-cgg-ctg-ctg |
| Group-specific PCRs | |
| Group A-forward | -gt-tTg-atc-mtg-gct-cag-rAc |
| Group B-forward | -gt-tTg-atc-mtg-gct-cag-aKtg |
| Group C-forward | agt-ttg-atc-mtg-gct-cag-gAt |
| Groups A, B, C-reverse (pD) | gta-tta-ccg-cgg-ctg-ctg |
| Group specific cycle sequencing reactions | |
| Common reverse (534R)* | tac-cgc-ggc-tgc-tgg-cac |