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Growth arrest of mcm2-1 cells with nearly 2C DNA at 38.5°C is reversed by the dbf4-6 mutation. 8534-8c (WT), 8534-M2 (mcm2-1) and mts2-1 (mcm2-1 dbf4-6) cells grown at 30°C or 38.5°C were stained with propidium iodide, and the DNA content of the cells were analyzed by flow cytometry. (A,C,E) DNA content profiles of the wild-type, mutant and suppressor strains at 30°C. (B,D,F) DNA content profiles of the three strains at 38.5°C.
