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. 1997 Dec 15;11(24):3365–3374. doi: 10.1101/gad.11.24.3365

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Copyright © 1997, Cold Spring Harbor Laboratory Press

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Mcm2, Mcm3, Mcm4, and Mcm6 are substrates of the Cdc7–Dbf4 kinase in vitro. The kinase assay was performed with Cdc7–Dbf4 protein kinase purified from Sf9 cells, and GST–Mcm proteins purified from yeast. The reactions were resolved in SDS–polyacrylamide gels for autoradiography (A), or for Coomassie brilliant blue staining (B). GST.Mcm2, (lane 1); GST.Mcm3, (lane 2); GST.Mcm4, (lane 3); GST.Mcm5, (lane 4); GST.Mcm6, (lane 5); GST.Mcm7, (lane 6); GST.Mcm2-1, (lane 7); GST.Mcm2, (lane 8); Mcm2, (lane 9).