Figure 5.
Acetylated H2A–H2B interacts with NAP-1. Coimmunoprecipitation of NAP-1 and H2A–H2B acetylated by p300 in the presence of Gal4–VP16. The second fractions from the top of the gradients shown in Figure 4B were analyzed by coimmunoprecipitation with preimmune serum and NAP-1 antiserum. The resulting immunoprecipitates were analyzed by 12% polyacrylamide-SDS gel electrophoresis followed by Western blotting for core histones (125I protein A detection method) and NAP-1 (enhanced chemiluminescent detection method).