Table 1. Plaque phenotype of parental EV71-6F and EV71-26M and virus chimeras on Vero cells.

Tenfold serial dilutions of virus were inoculated at a volume of 100 µl per well into 12-well tissue-culture trays. Infected cells were incubated for 7 days before staining with 0.05 % (w/v) crystal violet solution. See also Fig. 3.

Parental backbone virus	Virus	Mean diameter of plaques (mm)#	Plaque phenotype
EV71-6F	EV71-6F	<0.5	Small
	6F/5′UTR/26M	<0.5	Small
	6F/P1/26M	0.9 (0.5)*	Medium
	6F/NS/26M	1.4 (0.46)	Medium
EV71-26M	EV71-26M	2.29 (0.78)	Large
	26M/5′UTR/6F	2.09 (0.76)	Large
	26M/P1/6F	<0.5	Small
	26M/NS/6F	1 (0.47)	Medium

*No. in brackets is SD.