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. 2000 Aug 1;14(15):1872–1885.

Figure 2.

Figure 2

Nucleolar fragmentation and Sir complex redistribution is observed in generation 7 nmt1–451D but not NMT1 cells. Multilabel immunofluorescence study. Fixed NMT1 cells [average bud scar count (ABSC) = 7.3 ± 3.3] and nmt1–451D cells (ABSC = 6.9 ± 2.0) are stained blue with 4′,6′-diamidino-2-phenylindole (DAPI) to visualize nuclear DNA. Nucleoli are marked with antibodies to yeast fibrillarin (Nop1p, red). Sir3p appears green. (Left panels) DAPI plus Nop1p; (middle panels) DAPI plus Sir3p; (right panels) DAPI, Nop1p, and Sir3p. The NMT1 cell has an intact, crescent-shaped nucleolus and Sir3p appears as telomeric spots. Sir3p is redistributed to a fragmented nucleolus in the nmt1–451D cell (colocalization of Sir3p and Nop1p in the nucleolus is seen as yellow).