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. 2000 Aug 1;14(15):1872–1885.

Figure 4.

Figure 4

NMT1sip2Δ cells undergo rapid aging. (A) Sterility assay, performed as described in the legend to Fig. 1B. The shortened lifespan of these cells is accompanied by progressive sterility. (B) Multilabel immunohistochemical study of a sip2Δ cell at generation 12 (ABSC = 11.8 ± 3.6) showing Sir3p redistribution to a fragmented nucleolus. Note that 20%–25% of generation 12 sip2Δ cells exhibit these phenotypic changes (n = 1000 cells/scored per experiment; two independent experiments). (C) Increased ERC levels in sip2Δ cells. ABSC for the indicated generations were as follows: NMT1 (0.9 ± 1.1; 7.5 ± 2.8; 12.7 ± 4.1); NMT1sip2Δ (0.9 ± 1.1; 7.2 ± 2.3; 11.8 ± 3.6).