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. 2000 Aug 1;14(15):1872–1885.

Figure 8.

Figure 8

Figure 8

The cytoplasmic localization of a Mig1p–GFP fusion protein provides in vivo evidence for enhanced Snf1p kinase activity in sip2Δ cells. (A) Wild type and sip2Δ containing a CEN plasmid encoding Mig1p–GFP were sorted to the generations indicated. The intracellular distribution of Mig1p–GFP was then determined using fluorescence microscopy. Nomarski and fluorescence images of each field are shown. Arrows point to examples of cells where Mig–GFP is predominantly cytoplasmic. See text for further discussion. (B) Western blot of total cellular proteins isolated from young and old sip2Δ cells without (−) and with (+) a MIG1–GFP plasmid. Cells were recovered at the generational ages noted in parenthesis. The blot was probed with a GFP monoclonal antibody. The arrow points to the Mig1p–GFP fusion protein.