Figure 1.

Muscle histopathology. H&E staining of triceps or quadriceps femoris muscles in 8-week-old homozygous rmd mutant mice and unaffected (+/rmd or +/+) littermate controls (A–D) shows dystrophic changes including variation in fiber size, necrosis and regeneration of individual fibers and interstitial fibrosis. NADH-TR staining (E–H), immunostaining of mitochondrial outer membrane protein Tom20 and EM (M–P) show abnormal mitochondria. Mitochondria in rmd muscle fibers are enlarged and prominent at the periphery, but sparse in the center (I–L). z, Z line; m, M line; i, I band.