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. 2011 Jul 12;20(19):3841–3851. doi: 10.1093/hmg/ddr305

Figure 6.

Figure 6.

Mitochondrial degeneration in rmd. (A) EM of extensor digitorum longus muscle. In rmd, mitochondria are degraded by mitophagy. Scale bar = 0.5 μm. (B) Western blot of isolated muscle mitochondria immunodetected for Parkin, polyubiquitin, p62/SQSTM1 and LC3. TOM20, a mitochondrial outer membrane protein is used as loading control. Hindlimb mitochondria in rmd show significantly increased expression level in these mitophagy markers. (C) p62 and TOM20 immunohistrochemistry of hindlimb muscle section. Note that mitochondria are significantly enlarged and sparse in rmd. p62 colocalizes with the mitochondrial outer membrane protein TOM20. Polyubiquitin and mitochondrial protein cytochrome c oxidase (COX) colocalize. LC3 and TOM20 colocalize. Polyubiquitin and p62 colocalize. Scale bar = 10 μm.