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. 1998 May 15;12(10):1464–1473. doi: 10.1101/gad.12.10.1464

Figure 6.

Figure 6

Figure 6

Figure 6

 Atf1 acts as a nuclear anchor for Spc1. (A) The localization of Atf1 was determined by immunofluorescence performed on strain FG2156 in which the genomic atf1+ is replaced by a myc-tagged copy, and in the FG2157 (Δpcr1 and bearing the genomic myc-tagged atf1+). Cells were harvested before and after 10 min of KCl stress and fixed in cold methanol. The immunofluorescence was realized using the anti-myc antibody. (B) The strain GD1952 (Δatf1 and bearing the genomic myc-tagged copy of spc1+) and FG2158 (Δpcr1 and bearing the myc-tagged genomic spc1+) were grown to mid-log phase, and cells were harvested before and after KCl stress. For each time point, an aliquot was fixed in cold methanol for immunofluorescence analysis. The localization of Spc1 was determined with the anti-myc antibody. (C) The phosphorylation status of Spc1 in strains GD1952 and FG2158 was followed with the anti-p38P and the loading checked with the anti-myc antibodies, as described previously. Bar, 10 μm.