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Double-immunofluorescent labeling in the rat retina (a) and optic nerve (b, c) with anti-GFAP antibodies as marker for astrocytes showing that patient's autoantibodies are predominantly anti-astrocytic (asterisks and arrows) : serum (red), GFAP (green), and colocalization (yellow), nuclear labeling with DAPI (blue); ON optic nerve
