Fig. 4.

Location of methylated honeybee genes in the bimodal CpG ratio distribution. (Left) The CpG ratio distribution for honeybee genes (blue) is shown together with the distribution limited to just methylated genes (green). (Right) The cumulative distribution function for methylated genes is shown as a function of the CpG ratio. Although the majority of methylated genes lie in the lower mode of the bimodal distribution, there are a significant number of methylated genes that lie in the higher mode. The CpG ratio can misrepresent the methylation status; for instance, over 28% of methylated genes have a CpG ratio greater than 1. Methylation data were obtained from bisulfite sequencing (Lyko et al., 2010).