Figure 3.

 GFP–Pap1 localizes to the nucleus following oxidative stress. (A) A schematic of the GFP–pap1⁺ fusion construct expressed under the control of the intermediate strength nmt1⁺ promoter (pRep41). (B) Fluorescence microscopic analysis of untreated wild-type cells containing Rep41–GFP–Pap1, stained with DAPI to visualize the nucleus at 365 nm or at 390 nm to visualize the cellular distribution of GFP-tagged Pap1. (C) Fluorescence analysis of the same cells treated for 30 min with 0.2 mm H₂O₂.