Figure 6.

 Tyrosine phosphatase activity of CLR-1. (A) Full-length CLR-1 expressed in COS cells. (Solid bars) Amount of phosphate released from a Tyr-³²P-labeled RCML substrate by CLR-1 immunoprecipitates from COS cells transiently transfected with the wild-type clr-1 cDNA1 (CLR-1), the clr-1 cDNA1 harboring the C1013S mutation in the membrane-proximal phosphatase domain (C1013S), or the expression vector alone (Vector). (B) The phosphatase domain region of the intracellular domain of CLR-1 expressed in E. coli. Phosphate released was measured for GST fusions to the wild-type phosphatase domains (♦) and the identical protein bearing the C1013S mutation in the D1 domain (•). Error bars represent the standard errors of the mean.