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The A. thaliana cullin, AtCUL1, interacts with TIR1 in planta. (A) Sequence alignment of AtCUL1 with the human and S. cerevisiae cullins, Cul4A and Cdc53p, with PileUp (Genetics Computer Group, Inc., Madison,WI). Identical amino acids are boxed in black. Similar amino acids are boxed in gray. (B) Western blot analysis of crude seedling extracts (lanes 1–4) or α-myc immunoprecipitates (lanes 6–7).
