Figure 2. CB₂ agonist inhibits CXCL12-induced cell migration in breast cancer cells.

Breast cancer cells (A) MCF-7/CXCR4-WT and (B) MDA-MB231 (SCP2) were pretreated overnight with vehicle or JWH-015 (20 µM) and 1×10⁵ cells were plated on the top chamber of 8 µm pore polycarbonate membrane filters and medium in absence or presence of CXCL12 (100 ng/ml) was placed in the lower chamber. After 12 hours of incubation, cells that migrated across the filter towards medium with or without CXCL12 (100 ng/ml) were fixed, stained and counted by bright-field microscopy in five random fields. Data represent the mean ± SD, representative experiments (n = 3) are shown. *P<0.05 vs. vehicle. Veh: Vehicle.