Figure 3. Heat shock factor 1 (HSF1) deficiency abolishes the heat shock (HS)-synchronized circadian mPer2 rhythms and the heat shock element (HSE)-driven acute induction.

Wild-type (WT) and Hsf1 ^−/− mouse embryonic fibroblasts (MEFs) transfected with mPer2-Luc and HSE-SLR were treated with the (A) HS pulse or (B) dexamethasone (Dex). Circadian profiles were monitored by the real-time bioluminescence assay. Relative acute or normalized (deviation from moving average) profiles from each experiment (average values from 5 independent experiments) are shown.