Table 2. Physical characteristics and analysis parameters of proteins identified in trout ventricle using mass spectroscopy analysis of protein spots picked from 2 dimensional protein gels.
Protein name | gi# | MW (kDa) | pI | Score | Coverage % |
Cardiac myosin binding protein C | 172072588 | 142 | 6.33 | 55.47 | 2 |
Myomesin | 292616516 | 166 | 5.96 | 47.35 | 2 |
Actin, alpha | 4902905 | 42 | 5.23 | 175.59 | 32 |
Troponin I | 50540326 | 23 | 9.55 | 38.98 | 9 |
Troponin T, cardiac | 23097290 | 34 | 5.19 | 30.07 | 6 |
Troponin T, skeletal | 31377814 | 35 | 5.15 | 35.05 | 4 |
Myosin light Chain II | 29725603 | 19 | 4.73 | 39.62 | 8 |
Tropomyosin | 148231061 | 33 | 4.67 | 156.7 | 20 |
Note: gi#, accession number; MW, molar mass in kDa; pI, isoelectric point; Score, summed MS/MS search score; Coverage %, % of sequence covered by identified peptides.