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. 2011 Sep 7;6(9):e24484. doi: 10.1371/journal.pone.0024484

Figure 5. Dll1-expressing crypt cells are almost all postmitotic.

Figure 5

(A, B) Two possible models of the intestinal cell lineage tree. Our data favour the second model (B). (CP - common progenitor; SP - secretory progenitor; A - absorptive cell; S - secretory cell; G - goblet cell; E - enteroendocrine cell; P - Paneth cell). (C) Immunofluorescence staining for β-galactosidase protein (green) combined with staining for EdU incorporation (red) in crypts of Dll1+/lacZ mice, after a 1-hour pulse of EdU, with DRAQ5 nuclear staining in blue; almost all (93%) of the β-galactosidase-positive cells are EdU-negative. Arrowheads point to β-galactosidase+ EdU− cells; arrow points to a rare β-galactosidase+ EdU+ cell. (D, E) Immunofluorescence staining for the secretory cell markers Neurog3 (D, green) and Gfi1 (E, green), combined with staining for EdU incorporation (red). Even fewer of the cells expressing these markers of secretory specialization stain positive for EdU incorporation. Arrowheads point to nuclei positive for the secretory markers.