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. 2011 Sep 7;6(9):e24469. doi: 10.1371/journal.pone.0024469

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Xie et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Foxo3b inhibited β-catenin/T cell factor activity in 293T cell line. 293T cells were transfected with 3xTOPFlash, and the plasmids as indicated, together with pTK-renilla as an internal control; luciferase activity was measured after 24h. Date presented were the average (±SEM) of three independent experiments, performed in triplicate. “**” indicates p<0.01. (B) Foxo3b co-localized with β-catenin1/2. HeLa cells were transfected with GFP-β-catenin1 (B1-B3) or GFP-β-catenin2 (B4–B6), together with RFP-foxo3b. Transfected cells were then observed using fluorescent microscopy after 24h. (C) Foxo3b interacted with β-catenin1/2. 293T cells were transfected with the indicated expressing plasmids. HA-foxo3b was immunoprecipitated, and binding of Flag-β-catenin1 (C1) or Flag-β-catenin2 (C2) was analyzed by immunoblotting.