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. 2011 Sep 7;6(9):e24432. doi: 10.1371/journal.pone.0024432

Figure 5. Akt1 influences alveolar bud diameter and apoptosis incidence in adult mammary glands.

Figure 5

Martius yellow, soluble blue, brilliant crystal (MSB) stain of Akt1+/+ (A) and Akt1−/− (C) PND 70 mammary glands. Blue staining indicates collagen and purple staining indicates fibrin. TUNEL staining in Akt1+/+ (B) and Akt1−/− (D) PND 70 mammary glands. Measurement of alveolar bud diameter in Akt1+/+ and Akt1−/− PND 70 mammary glands (E). Measurement of TUNEL positivity in Akt1+/+ and Akt1−/− PND 70 mammary glands (F). Arrow indicates apoptotic cells in the Akt1−/− gland. Scale bars indicate 100 µm. Data represented as mean plus standard error. Statistical analyses were conducted using a two-tailed student's t test. #p = 0.08, *p<0.05. n = 4 per genotype for (E), n = 3 per genotype for (F).