Table 1.
Comparison of protein surface coverages achieved on different gold substrates using different immobilization conditions
| Protein | Substrate | Activating molecule | Immobilization method | Incubation time (min) | Protein coverage (protein/μm2) |
|---|---|---|---|---|---|
| aBSA | rough gold | EDC | static | 1 | 491 (3) |
| 10 | 590 (8) | ||||
| 15 | 658 (11) | ||||
| aBSA | rough gold | NHS-ester | static | 1 | 328 (5) |
| 10 | 355 (10) | ||||
| 15 | 398 (13) | ||||
| BSA | NPG | NHS-ester | flow | 120 | 627 (36) (exterior coverage) |
| IgG | NPG | NHS-ester | flow | 120 | 368 (14) (exterior coverage) |
a
Protein coverages reported here on rough gold represent values accounting for the roughness factor of 2.5 found for rough gold. The values in parenthesis are the plausible numbers of additional proteins identified by image analysis as present in aggregates or possibly subject to tip convolution.