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. Author manuscript; available in PMC: 2011 Nov 26.
Published in final edited form as: Neuron. 2011 May 26;70(4):742–757. doi: 10.1016/j.neuron.2011.04.002

Figure 2. CYY-1 Is Required for the Completion of DD Synaptic Remodeling.

Figure 2

(A) Schematic of experimental timeline. Experiments were performed at 25 °C. See experimental procedures for details. White box denotes the L1 stage. See also Figure S1.

(B) cyy-1 mutants exhibit incomplete synaptic remodeling. Note that cyy-1 mutants still have significant amount of GFP::RAB-3 in the ventral processes at 26-hr (B6). Scale bars, 20 µm.

(C) Overexpression of CYY-1 accelerates DD synaptic remodeling. Note the weak ventral GFP::RAB-3 signal at 16-, 19.5-, and 22-hr in worms overexpressing CYY-1 (C4–C6). Scale bars, 20 µm.

(D) Quantification for appearance of dorsal GFP::RAB-3 and disappearance of ventral GFP::RAB-3. The X axis indicates the time points since egg-laying. White, gray, and black indicate worms showing GFP::RAB-3 signals only in the ventral (only V), both in the ventral and dorsal (V+D), and only in the dorsal (only D) sides, respectively, regardless of their intensity. wyEx2844 is Ex[DD::CYY-1] in wild-type background, and wyEx2889 is Ex[DD::CYY-1] in cyy-1 mutant background. *p<0.05, **p<0.005, ns, not significant relative to wild-type; #p<0.05, ##p<0.005, NS, not significant relative to cyy-1, Fisher’s exact test. Symbols for statistics are labeled inside of the gray-filled and above the black-filled bars for the phenotypes of “V+D” and “only D”, respectively.

(E, F, and G) Average intensity quantification for the ratio of dorsal to ventral plus dorsal [D/(V+D)] GFP::RAB-3 (E), for ventral GFP::RAB-3 (F), and for dorsal GFP::RAB-3 (G) from Figures 2B and 2C. Data represent means ± SEM. *p<0.05, **p<0.005 relative to wild-type, student t-test. See the Supplemental Data for n numbers. See also Figure S4.