Table 3.
Mitochondrial oxidative capacities after 30 min of normoxic perfusion
| Respiratory state | Young adults | Middle aged | ANOVA | |
|---|---|---|---|---|
| GM | Substrate | 23 ± 1 | 20 ± 1 | NS |
| +ADP | 153 ± 6 | 131 ± 15 | NS | |
| +Oligomicine | 26 ± 3 | 33 ± 6 | NS | |
| +DNP | 219 ± 14 | 183 ± 26 | NS | |
| RCR | 6.43 ± 0.68 | 4.28 ± 0.38 | p < 0.05 | |
| S | Substrate | 70 ± 4 | 78 ± 4 | NS |
| +ADP | 239 ± 16 | 276 ± 14 | NS | |
| +Oligomicine | 70 ± 4 | 81 ± 5 | NS | |
| +DNP | 214 ± 15 | 246 ± 11 | NS | |
| RCR | 3.46 ± 0.04 | 3.45 ± 0.19 | NS | |
| GMS | Substrate | 59 ±4 | 67 ± 3 | NS |
| +ADP | 263 ± 11 | 245 ± 31 | NS | |
| +Oligomicine | 74 ± 4 | 80 ± 7 | NS | |
| +DNP | 259 ± 21 | 279 ±13 | NS | |
| RCR | 3.33 ±0.13 | 3.57 ± 0.27 | NS |
Except the RCR which is the ratio between the state III and the state IV respiration rates, the results are expressed in ng atoms of oxygen/min/mg of proteins. The number of experiments was eight per group
GM glutamate (5 mM) + malate (2.5 mM), S succinate (5 mM) + rotenone (2.5 μM), GMS glutamate (5 mM) + malate (2.5 mM) + succinate (5 mM), substrate state II respiration rate, +ADP state III respiration rate, +oligomicine state IV respiration rate, +DNP state uncoupled by dinitrophenol, RCR respiratory complex ratio