Fig. 3.

Bone changes in female and male WT and TTD mice, aged 52 and 104 weeks. a Trabecular bone volume fraction (Trab BV/TV) in tibial epiphysis. b Trab BV/TV in tibial metaphysic. c Subchondral bone plate thickness in the tibial epiphysis. Please note that values refer to the average thickness of the medial and lateral subchondral bone plate. d Thickness of non-calcified (NCC) and calcified (CC) portion of the cartilage layer. Please note the decreased values and increased standard error in both sexes of the 104-week-old WT mice. This was caused due to the severe cartilage damage in two cases (one mouse in each group), for which it was not possible to obtain a thickness value. In these cases ‘0’ was used as value. The histological section (magnification ×200) is provided for anatomical context, the dashed line indicates the border between NCC and CC. Shown are averages ±SEM, significance was tested within genders, with p < 0.05 considered significant. Ages are depicted inside the bars. NCC non-calcified cartilage, CC calcified cartilage, SBP subchondral bone plate, BM bone marrow