Fig. 5.

Aging was associated with an increase in IL-6 immunostaining in kidneys. a Immunohistochemical (IHC) analysis of IL-6 expression in renal cortex. Brown staining (arrows) indicated positive IL-6 expression. IL-6 expressed in and around the nucleus. Negative control, without the first antibody (400×). b Semi-quantification of IL-6 expression in renal cortex. c IHC analysis of IL-6 expression in renal medulla. Brown staining (arrows) indicated positive IL-6 expression. IL-6 expressed in the basal membrane of renal tubules. Negative control, without the first antibody (400×). d Semi-quantification of IL-6 expression in renal medulla. Data = mean ± SE. ***p < 0.001 versus the Young Intact group; addition symbol, p < 0.05, double addition symbols, p < 0.01 versus the Old Impaired group. N = 11 animals in the Old Intact group, n = 10 animals in the young intact group, n = 9 animals in the Old Impaired group