Fig. 8.

MnSOD expression was downregulated in kidneys of the Old Impaired rats. a Representative Western blot bands and quantitative analysis of MnSOD protein expression in renal cortex. b Representative Western blot bands and quantitative analysis of MnSOD protein expression in renal medulla. c IHC analysis of MnSOD expression in renal medulla. Brown staining indicated positive MnSOD protein expression. MnSOD expressed in renal tubule epithelial cells. Negative control, without the first antibody (200×). d Semi-quantification of MnSOD expression in renal medulla. Data = mean ± SE. *p < 0.05, **p < 0.01, ***p < 0.001 versus the young intact group; addition symbol, p < 0.05 vs the Old Impaired group. N = 11 animals in the Old Intact group, n = 10 animals in the Young Intact group, n = 9 animals in the Old Impaired group