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eRF1 and eRF3 inhibit Upf1p RNA-dependent ATPase activity. Upf1p RNA-dependent ATPase activity was determined in the presence of GST–RF fusions by a charcoal assay with 1 μg/ml poly(U) RNA and 100 μg/ml BSA. The results are plotted as picomoles of 32P released vs. the amount of the indicated protein. (○) GST; (□) GST–eRF3; (▵) GST–eRF1.
