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Biochemical purification Vg1 RBP. (a) Fractions of the heparin–Sepharose column were assayed for Vg1 RBP cross-linking activity. KCl concentrations (as determined by conductivity) of selected fractions are indicated. (b) Equivalent fractions of the RNA affinity column were assayed for Vg1 RBP cross-linking activity (autoradiograph, top) and presence of protein (silver-stained gel, bottom). (Arrows) The location of Vg1 RBP.
