Skip to main content
. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 17;50(36):7774–7786. doi: 10.1021/bi200847n

Figure 6. Mobilization of calcium is essential and sufficient for the cleavage of vimentin.

Figure 6

HEL cells were first pretreated with either 30 μM verapamil for 4 hours (A) or 10 μM BAPTA-AM for 2 hours (B) and then treated with 30 μM G6 for 24 hours. Post treatment, the cells were lysed, proteins were separated by gel electrophoresis and then immunoblotted with an anti-vimentin antibody (upper panel) or an anti-β-actin antibody (lower panel) to confirm equal protein across all lanes. (C) HEL cells were treated with 10 μM A23187 for the indicated periods of time. Cellular lysates were then probed with an anti-vimentin antibody (top panel). An anti-β-actin antibody was used as a loading control (bottom panel). Shown is a representative blot from three independent experiments for each.