Figure 8. G6 treatment decreases the levels of vimentin protein, in vivo.
NOD/SCID mice were randomized into 5 groups (n=6 per group). One group consisted of naïve animals that did not receive any treatment whatsoever. All other mice received 2 × 106 HEL cells via a single tail vein injection. Three weeks after injection, the mice began receiving vehicle control solution (DMSO) or G6 at doses of 0.1, 1, and 10 mg/kg/day. After three weeks of treatment, the mice were euthanized. Anti-vimentin immuno-histochemistry was then carried out on bone marrow sections from the indicated groups of animals. Shown are representative stained bone marrow sections from each treatment group at 40X (A) and 100X (B) magnifications. Also shown is a negative control IgG antibody used in place of the anti-vimentin primary antibody in the immuno-histochemical procedure.