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. 1998 Jun 1;12(11):1638–1651. doi: 10.1101/gad.12.11.1638

Figure 5.

Figure 5

Figure 5

 PCAF domain analysis and the relationship with p300. (A) Heterodimer–RARE complexes (∼2 pmoles) were incubated with 2 pmoles of recombinant N-p300 (amino acids 1–670) alone (lanes 1,2) or along with 1 (+) and 2 (++) pmoles of PCAF (lane 3–6) in the presence (+) or absence (−) of 1 μm 9-cis RA. (B) PCAF domain analysis: 2 pmoles of rPCAF lacking the carboxy-terminal domain (ΔC) or amino-terminal domain (ΔN1 and ΔN2, top) were incubated with heterodimer–RARE complexes (1 μg of heterodimer) in the presence (+) or absence (−) of 1 μm 9-cis RA. Binding was detected with anti-PCAF antibody.