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. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 17;50(36):7765–7773. doi: 10.1021/bi2005784

Fig. 2. Accumulation of nuclear Nrf2 in cells treated with NA.

Fig. 2

Cells were treated for 3 hr with 10 μM total NO2-LA, 9/10-NO2-OA, or SFN (positive control). Cells were incubated for variable time periods after removing the inducing agent and harvested for preparation and Western blotting of nuclear protein 5, 10, and 21 hr after the start of NA or SFN induction (40) using antibodies directed against Nrf2 or topoisomerase I loading control.