Cells were transfected with the ARE-luciferase reporter gene according to transfection scheme 1. One hr prior to the addition of 10 μM inducing agent (total NO2-LA, 9/10-NO2-OA, or SFN) or vehicle control, cells were treated with PI3K inhibitors 0.5 μM wortmannin (black bars) or 25 μM LY294002 (cross-hatched bars), or no inhibitor (open bars). Inhibitors remained in the medium throughout the subsequent 3 hr induction and until harvest 10 hr after the start of induction. Data was corrected for variations in transfection efficiencies and expressed as % maximum fold induction of control (minus inhibitors, open bars). Error bars: ± 1 sd (n=3).