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. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 17;50(36):7765–7773. doi: 10.1021/bi2005784

Fig. 4. PI3K activity is required for full activation of ARE/Nrf2-dependent transcription by NAs.

Fig. 4

Cells were transfected with the ARE-luciferase reporter gene according to transfection scheme 1. One hr prior to the addition of 10 μM inducing agent (total NO2-LA, 9/10-NO2-OA, or SFN) or vehicle control, cells were treated with PI3K inhibitors 0.5 μM wortmannin (black bars) or 25 μM LY294002 (cross-hatched bars), or no inhibitor (open bars). Inhibitors remained in the medium throughout the subsequent 3 hr induction and until harvest 10 hr after the start of induction. Data was corrected for variations in transfection efficiencies and expressed as % maximum fold induction of control (minus inhibitors, open bars). Error bars: ± 1 sd (n=3).