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Restoration of cAMP oscillations in Ddmyb2-null cells by ectopic expression of ACA. Cells were starved for 5 hr, washed, and resuspended to a density of 108 cell/ml. Samples of the cell suspension were taken at 1-min intervals and used to determine the cAMP concentration. (A) Wild-type Ax3; (B) Ddmyb2–null cells (○), Ddmyb2−/ACA cells (•). The results shown are from a typical experiment reproduced two times.
